CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30149APC

Discontinued Product
NBP2-30149APC has been discontinued. View all CD3 epsilon products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Cell depletion, T Cell Stimulation, CyTOF-ready

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 145-2C11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Reactivity Notes

Mouse.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Applications for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

Application
Recommended Usage

Cell depletion

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

T Cell Stimulation

Optimal dilutions of this antibody should be experimentally determined.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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