Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # IGEL/773

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD20 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD20 protein (Uniprot: P11836 )

Reactivity Notes

Others not known.

Localization

Predominantly cell surface with some cytoplasmic

Specificity

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This monoclonal antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide.

Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD20 Antibody (IGEL/773) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Immunocytochemistry/Immunofluorescence: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840] - Immunofluorescence staining of Raji cells using CD20 Antibody (IGEL/773) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840] - Human Lymphoma stained with CD20 Monoclonal Antibody (IGEL/773)

Flow Cytometry: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Flow Cytometry: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840] - Flow Cytometric Analysis of Raji cells. CD20 Antibody (IGEL/773) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Applications for CD20 Antibody (IGEL/773) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml

Application Notes

Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD20

CD20 is a non-glycosylated phosphoprotein that is expressed on the surface of normal and malignant B cells and functions in mediating calcium transport and B cell differentiation (1,2). CD20 is encoded by the membrane-spanning 4-domain family A member 1 (MS4A1) gene and, in humans, is located on chromosome 11q12 (1). The CD20 protein is 297 amino acids (aa) in length with a theoretical molecular weight (MW) of 33 kDa (1,2). Structurally, the CD20 protein has four membrane-spanning domains, two extracellular loop domains, and intracellular N- and C-terminal domains (1,2). CD20 is expressed at specific stages of B cell maturation including pre-B cells, mature naive and activated B cells, and memory B cells, but is absent from plasmablasts and plasma cells (1,3,4). Expression of CD20 is often increased on malignant B cells associated with various B cell disorders such as chronic lymphocytic leukemia (CLL), multiple sclerosis (MS), and rheumatoid arthritis (2-4). Anti-CD20 monoclonal antibody (mAb)-based therapies have become an appealing target for treating these immune-related disorders and cancers (1-5). Rituximab, a chimeric mAb, was the first FDA approved CD20 monoclonal antibody for the treatment of non-Hodgkin's lymphoma that is now commonly used to treat MS (2,3). Since its initial approval in 1997, several other chimeric and humanized anti-CD20 mAbs have been developed including Ofatumumab, Ublituximab, and Obinutuzumab (1-5). B cell depletion via CD20 mAbs can occur under different mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), complement-dependent cytotoxicity (CDC), and direct induction of apoptosis (1-4). Many ongoing studies are focused on combination therapies with CD20 mAbs as an addition to chemotherapy, B cell receptor (BCR) signaling inhibitors, or BH3 mimetics (1,2,4). Additionally, the effects of bispecific antibodies and CD20 chimeric antigen receptor (CAR) T cell therapies are under investigation for the treatment of B cell malignancies (4,5).

References

1. Pavlasova G, Mraz M. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020; 105(6):1494-1506. https://doi.org/10.3324/haematol.2019.243543

2. Payandeh Z, Bahrami AA, Hoseinpoor R, et al. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomed Pharmacother. 2019; 109:2415-2426. https://doi.org/10.1016/j.biopha.2018.11.121

3. Margoni M, Preziosa P, Filippi M, Rocca MA. Anti-CD20 therapies for multiple sclerosis: current status and future perspectives. J Neurol. 2022; 269(3):1316-1334. https://doi.org/10.1007/s00415-021-10744-x

4. Klein C, Jamois C, Nielsen T. Anti-CD20 treatment for B-cell malignancies: current status and future directions. Expert Opin Biol Ther. 2021; 21(2):161-181. https://doi.org/10.1080/14712598.2020.1822318

5. Sharman JP. Targeting CD20: teaching an old dog new tricks. Hematology Am Soc Hematol Educ Program. 2019; 2019(1):273-278. https://doi.org/10.1182/hematology.2019000031

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for CD20 Antibody (IGEL/773) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD20 Antibody (IGEL/773) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD20 Antibody (IGEL/773) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47840

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CD20 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Specificity

Marker

Clonality

Host

Isotype

Description

Scientific Data Images for CD20 Antibody (IGEL/773) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Flow Cytometry: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - Azide and BSA Free [NBP2-47840]

Applications for CD20 Antibody (IGEL/773) - Azide and BSA Free

Flow Cytometry

Immunofluorescence

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD20

Long Name

Alternate Names

Gene Symbol

Additional CD20 Products

Product Documents for CD20 Antibody (IGEL/773) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD20 Antibody (IGEL/773) - Azide and BSA Free