CD117/c-kit Antibody (2B8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-77477
![Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]")
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Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # 2B8
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Mouse bone marrow mast cells
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 23967247).
Marker
Hematopoietic Stem Cell Marker
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images for CD117/c-kit Antibody (2B8) - BSA Free
Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]
Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).![Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]")
Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]
CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg![Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0002.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]")
Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]
Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.Applications for CD117/c-kit Antibody (2B8) - BSA Free
Application
Recommended Usage
Flow Cytometry
0.6 ug/10^6 cells in 100 uL
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 28097227)
Immunohistochemistry
1:20 - 1:1000
Immunohistochemistry-Frozen
1:20 - 1:1000
Immunoprecipitation
1:20 - 1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
Reviewed Applications
Read 1 review rated 1 using NB100-77477 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (2B8) - BSA Free
Product Specific Notices for CD117/c-kit Antibody (2B8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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