CCRL2/CRAM-A/B Antibody (BZ5B8) [PerCP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11981PCP
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional Assay
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # BZ5B8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was raised by immunising Wistar Furth rats with the mCCRL2/CRAM-A/B peptide/KLH conjugate. The immunizing amino-terminal mCCRL2/CRAM-A/B peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2/CRAM-A/B, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Specificity
This antibody is specific for murine CCRL2/CRAM-A/B (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2/CRAM-A/B presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2/CRAM-A/B antibody binding.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for CCRL2/CRAM-A/B Antibody (BZ5B8) [PerCP]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional Assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP3-11981. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CCRL2/CRAM-A/B
Long Name
CC Chemokine Receptor-like 2
Alternate Names
ACKR5, CCRL2, CKRX, CRAM-A, CRAM-B, CRAMA, CRAMB, HCR
Gene Symbol
CCRL2
Additional CCRL2/CRAM-A/B Products
Product Documents for CCRL2/CRAM-A/B Antibody (BZ5B8) [PerCP]
Product Specific Notices for CCRL2/CRAM-A/B Antibody (BZ5B8) [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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