Caspase-2 Antibody (3D4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26465
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3D4 expressed in HEK293
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Caspase-2 [UniProt P42575]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Caspase-2 Antibody (3D4) - BSA Free (NBP3-26465) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Caspase-2 Antibody (3D4) - BSA Free
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] -
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] - Image of Caspase-2 Antibody (3D4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] -
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] - Image of Caspase-2 Antibody (3D4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: Caspase-2 Antibody (3D4) [NBP3-26465] -
Western Blot: Caspase-2 Antibody (3D4) [NBP3-26465] - Positive Western Blot detected in: K562 whole cell lysate, 293 whole cell lysate.All lanes: Caspase-2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 51, 35, 11 kDa
Observed band size: 51 kDa
Applications for Caspase-2 Antibody (3D4) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Caspase-2
Caspase-2, also known as NEDD2 and ICH-1, is a 30‑32 kDa member of the peptidase C14A/IL‑1 beta‑converting family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-2 is a disulfide-linked homodimer, with activation occurring following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome. Initially, Pro-Caspase-2 is cleaved into a p34 subunit and a p14 subunit which are further processed to generate the prodomain, plus the mature p18 and p12 subunits. Notably, each p18-p12 heterodimer demonstrates proteolytic activity and localizes to either the nucleus or cytoplasm.
Alternate Names
CASP2, Caspase2, ICH-1, NEDD2
Gene Symbol
CASP2
Additional Caspase-2 Products
Product Documents for Caspase-2 Antibody (3D4) - BSA Free
Product Specific Notices for Caspase-2 Antibody (3D4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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