Casein Kinase 2 alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-377
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0005.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]")
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0012.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]")
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0011.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Chicken (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative), Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Casein Kinase 2 alpha Antibody
Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]
Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0013.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]")
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for Casein Kinase 2 alpha Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 -1:2000
Immunohistochemistry-Paraffin
1:500 -1:2000
Western Blot
1:2000 - 1:10000
Application Notes
By WB, this antibody recognizes a band at ~42 kDa, representing CKII alpha. Not recommended for immunoprecipitation. *NB 100-378 is recommended for IP. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-buffered Saline, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Casein Kinase 2 alpha
Additional Casein Kinase 2 alpha Products
Product Documents for Casein Kinase 2 alpha Antibody
Product Specific Notices for Casein Kinase 2 alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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