Casein Kinase 2 alpha Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-376
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0016.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]")
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0022.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Chicken (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation (Negative), Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-171, NB100-97831
Scientific Data Images for Casein Kinase 2 alpha Antibody - BSA Free
Western Blot: Casein Kinase 2 alpha Antibody [NB100-376]
Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Casein Kinase 2 alpha Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:5000
Application Notes
Not recommended for immunoprecipitation (use NB 100-378 for IP). WB of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Casein Kinase 2 alpha
Additional Casein Kinase 2 alpha Products
Product Documents for Casein Kinase 2 alpha Antibody - BSA Free
Product Specific Notices for Casein Kinase 2 alpha Antibody - BSA Free
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-171, NB100-97831
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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