CARS Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86624

Novus Biologicals, part of Bio-Techne
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NBP1-86624
NBP1-86624-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CARS Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARS Antibody - BSA Free

CARS Antibody - BSA Free Immunohistochemistry-Paraffin: CARS Antibody - BSA Free [NBP1-86624]

Immunohistochemistry-Paraffin: CARS Antibody - BSA Free [NBP1-86624]

Staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624]

Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624]

Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining in human cerebral cortex and skeletal muscle tissues using anti-CARS antibody. Corresponding CARS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624]

Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624]

Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex shows high expression.
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Applications for CARS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARS

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

CARS1, CysRS, cysteine translase, cysteine tRNA ligase 1, cytoplasmic, Cysteine--tRNA ligase, cysteinyl-tRNA synthetase, cysteinyl-tRNA synthetase, cytoplasmic, EC 6.1.1, EC 6.1.1.16, MGC:11246

Gene Symbol

CARS1

Additional CARS Products

Product Documents for CARS Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CARS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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