Bromodeoxyuridine/BrdU Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-235
![Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-Immunocytochemistry-Immunofluorescence-NB500-235-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, All Species, Guinea Pig
Cited:
Human, Mouse, Rat, Guinea Pig, Rabbit
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH).
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33731750).
Specificity
BrdU
Marker
Proliferation Marker
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody - Azide and BSA Free
![Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-Immunoprecipitation-NB500-235-img0005.jpg "Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]")
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235] - Lysate was immunoprecipitated with anti-brdu antibody (NB500-235), 5-methyl cytosine (5-Me), or a no antibody control (No A). An ELISA was then performed to measure total activity.Applications for Bromodeoxyuridine/BrdU Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
25-100 ug/ml
Application Notes
Use in IHC-Fr reported in scientific literature (PMID:33731750). Use in IHC-FrFl reported in scientific literature (PMID:33744708) In vitro reported in (PMID: 29100301).
Reviewed Applications
Read 2 reviews rated 5 using NB500-235 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody - Azide and BSA Free
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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