Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81093
Recombinant Monoclonal Antibody
![ELISA: Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free [NBP2-81093]](https://resources.bio-techne.com/images/products/Birch-pollen-Bet-V1-Antibody-BV16-ELISA-NBP2-81093-img0001.jpg "ELISA: Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free [NBP2-81093]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Plant
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # BV16
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Birch pollen Bet V1-A, a major allergen of the European white birch.
Reactivity Notes
Birch (Betula pendula)
Specificity
This antibody binds to Birch pollen Bet V1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free
ELISA: Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free [NBP2-81093]
ELISA: Birch pollen Bet V1 Antibody (BV16) [NBP2-81093] - Binding curve of [NBP2-81093] to Bet v 1. ELISA Plate coated with Bet v 1-A at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81093. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more. This is suggestive of two binding sites on Bet v 1-A for which NBP2-81093 has differing affinities.Applications for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Bet v 1 via an epitope commonly used by allergogenic IgE. Fab versions of this antibody have been used in co-crystallization studies of Bet v 1.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Birch pollen Bet V1
Alternate Names
Allergen Bet v I-A, Allergen=Bet v 1-A, BETVI, BETVIA, Major pollen allergen Bet v 1-A
Additional Birch pollen Bet V1 Products
Product Documents for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free
Product Specific Notices for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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