BIN1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46170
![Immunoprecipitation: BIN1 Antibody [NBP1-46170]](https://resources.bio-techne.com/images/products/BIN1-Antibody-Immunoprecipitation-NBP1-46170-img0008.jpg "Immunoprecipitation: BIN1 Antibody [NBP1-46170]")
![Western Blot: BIN1 Antibody [NBP1-46170]](https://resources.bio-techne.com/images/products/BIN1-Antibody-Western-Blot-NBP1-46170-img0007.jpg "Western Blot: BIN1 Antibody [NBP1-46170]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 543 and 593 of human Bridging Integrator 1 using the numbering given in entry NP_647593.1 (GeneID 274).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit BIN1 Antibody - BSA Free (NBP1-46170) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BIN1 Antibody - BSA Free
Western Blot: BIN1 Antibody [NBP1-46170]
Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170]](https://resources.bio-techne.com/images/products/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-46170-img0005.jpg "Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170]")
Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170]
Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Bin1 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .![Western Blot: BIN1 Antibody [NBP1-46170]](https://resources.bio-techne.com/images/products/BIN1-Antibody-Western-Blot-NBP1-46170-img0001.jpg "Western Blot: BIN1 Antibody [NBP1-46170]")
Western Blot: BIN1 Antibody [NBP1-46170]
Western Blot: BIN1 Antibody [NBP1-46170] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Bin1 was also immunoprecipitated by rabbit anti-Bin1 antibody NBP1-46169, which recognizes an upstream epitope.Applications for BIN1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:1000
Immunoprecipitation
5-15 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BIN1
Alternate Names
AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9
Gene Symbol
BIN1
Additional BIN1 Products
Product Documents for BIN1 Antibody - BSA Free
Product Specific Notices for BIN1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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