beta Tubulin Antibody (A1-A4-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32081

Recombinant Monoclonal Antibody
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NBP3-32081
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A1-A4-R expressed in HEK293

Concentration

1 mg/ml

View all beta Tubulin Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human beta Tubulin aa 151-200 / 444. (Uniprot: P07437)

Localization

Cytoplasm, Cytoskeleton, Microtubule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Tubulin Antibody (A1-A4-R)

beta Tubulin Antibody (A1-A4-R)

Immunohistochemistry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081]

Immunohistochemistry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32081) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
beta Tubulin Antibody (A1-A4-R)

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (A1-A4-R) [NBP3-32081]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin Antibody (A1-A4-R)

Flow Cytometry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081]

Flow Cytometry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Flow cytometric analysis of HeLa cells labeling beta Tubulin.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32081, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Applications for beta Tubulin Antibody (A1-A4-R)

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:10000

Western Blot

1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody (A1-A4-R)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta Tubulin Antibody (A1-A4-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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