BDNF Antibody (23GB3005)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52007

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NBP3-52007-100ul
NBP3-52007-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3005

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BDNF Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human BDNF (Uniprot: P23560)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BDNF Antibody (23GB3005)

BDNF Antibody (23GB3005)

Immunocytochemistry/ Immunofluorescence: BDNF Antibody (23GB3005)[NBP3-52007] -

Immunocytochemical staining of HepG2 cells with BDNF antibody (1:1,000). Nuclei were stained blue with DAPI; Brain derived neurotrophic factor was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
BDNF Antibody (23GB3005)

Flow Cytometry: BDNF Antibody (23GB3005)[NBP3-52007] -

Flow cytometric analysis of BDNFexpression in HepG2 cells using Brain derived neurotrophic factor antibody (1:2,000). Green, isotype control; red, Brain derived neurotrophic factor.
BDNF Antibody (23GB3005)

Western Blot: BDNF Antibody (23GB3005)[NBP3-52007] -

Western blotting analysis using BDNF antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
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Applications for BDNF Antibody (23GB3005)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BDNF

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family. BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Gene Symbol

BDNF

Additional BDNF Products

Product Documents for BDNF Antibody (23GB3005)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BDNF Antibody (23GB3005)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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