Bcl-6 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76541
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EHVVDTCRKFIKASEAEMVSAIKPPREEFLNSRMLMPQDIMAYRGREVVENNLPLRSAPGCESRAFAPSLYSGLSTPPASYSMYSHLPVSSLLFSDEEFRDVR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Bcl-6 Antibody - BSA Free
![Western Blot: Bcl-6 Antibody [NBP2-76541]](https://resources.bio-techne.com/images/products/Bcl-6-Antibody-Western-Blot-NBP2-76541-img0001.jpg "Western Blot: Bcl-6 Antibody [NBP2-76541]")
Western Blot: Bcl-6 Antibody [NBP2-76541]
Western Blot: Bcl-6 Antibody [NBP2-76541] - Analysis in human cell line RT-4 and human cell line U-251 MG.![Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-76541]](https://resources.bio-techne.com/images/products/Bcl-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76541-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-76541]")
Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-76541]
Immunocytochemistry/Immunofluorescence: Bcl-6 Antibody [NBP2-76541] - Staining of human cell line RT4 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.![Bcl-6 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Bcl-6 Antibody - BSA Free [NBP2-76541]](https://resources.bio-techne.com/images/products/nbp2-76541_rabbit-polyclonal-bcl-6-antibody-25620259121980.jpg "Chromatin Immunoprecipitation-exo-Seq: Bcl-6 Antibody - BSA Free [NBP2-76541]")
Chromatin Immunoprecipitation-exo-Seq: Bcl-6 Antibody - BSA Free [NBP2-76541]
ChIP-Exo-Seq composite graph for Anti-BCL6 (NBP2-76541) tested in HepG2 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for Bcl-6 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Bcl-6
Long Name
B Cell Lymphoma/Leukemia 6
Alternate Names
Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51
Gene Symbol
BCL6
Additional Bcl-6 Products
Product Documents for Bcl-6 Antibody - BSA Free
Product Specific Notices for Bcl-6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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