BBOX1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92462
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human BBOX1 (NP_003977.1). MACTIQKAEALDGAHLMQILWYDEEESLYPAVWLRDNCPCSDCYLDSAKARKLLVEALDVNIGIKGLIFDRKKVYITWPDEHYSEFQADWLKKRCFSKQARAKLQRELFFPECQYWGSELQLPTLDFEDVLRYDEHAYKWLSTLKKVGIVRLTGASDKPGEVSKLGKRMGFLYLTFYGHT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit BBOX1 Antibody - BSA Free (NBP2-92462) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BBOX1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody - BSA Free [NBP2-92462] -
Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody - BSA Free [NBP2-92462] - Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse kidney cells using BBOX1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: BBOX1 Antibody - BSA Free [NBP2-92462] -
Western Blot: BBOX1 Antibody - BSA Free [NBP2-92462] - Western blot analysis of extracts of various cell lines, using BBOX1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Applications for BBOX1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BBOX1
Alternate Names
2-oxoglutarate dioxygenase, 2-oxoglutarate dioxygenase 1, BBHgamma-butyrobetaine, BBOXEC 1.14.11.1, butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1, gamma-BBH, Gamma-butyrobetaine, gamma-butyrobetaine dioxygenase, Gamma-butyrobetaine hydroxylase, G-BBH
Gene Symbol
BBOX1
Additional BBOX1 Products
Product Documents for BBOX1 Antibody - BSA Free
Product Specific Notices for BBOX1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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