B220/CD45R Antibody (PTPRC/1783R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53303
Recombinant Monoclonal Antibody
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0005.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
![Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Western-Blot-NBP2-53303-img0006.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PTPRC/1783R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human full-length B220/CD45R protein (Uniprot: P08575 )
Localization
Cell Surface and Cytoplasmic
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for B220/CD45R Antibody (PTPRC/1783R)
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).![Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunocytochemistry-Immunofluorescence-NBP2-53303-img0003.jpg "Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0004.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0001.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0002.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Applications for B220/CD45R Antibody (PTPRC/1783R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-53303 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: B220/CD45R
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (PTPRC/1783R)
Product Specific Notices for B220/CD45R Antibody (PTPRC/1783R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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