ATP5D Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86162
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PNQMSFTFASPTQVFFNGANVRQVDVPTLTGAFGILAAHVPTLQVLRPGLVVVHAEDGTTSKYFVSSGSIAVNADSSVQLLAEEAVTLDMLDLGAAKANLEKAQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ATP5D Antibody - BSA Free (NBP1-86162) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ATP5D Antibody - BSA Free
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-86162]](https://resources.bio-techne.com/images/products/ATP5D-Antibody-Immunohistochemistry-Paraffin-NBP1-86162-img0001.jpg "Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-86162]")
Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-86162]
Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-86162] - Staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.![ATP5D Antibody - BSA Free Western Blot: ATP5D Antibody - BSA Free [NBP1-86162]](https://resources.bio-techne.com/images/products/nbp1-86162_rabbit-polyclonal-atp5d-antibody-24420259342994.jpg "Western Blot: ATP5D Antibody - BSA Free [NBP1-86162]")
Western Blot: ATP5D Antibody - BSA Free [NBP1-86162]
Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
![ATP5D Antibody - BSA Free Western Blot: ATP5D Antibody - BSA Free [NBP1-86162]](https://resources.bio-techne.com/images/products/nbp1-86162_rabbit-polyclonal-atp5d-antibody-24420259374118.jpg "Western Blot: ATP5D Antibody - BSA Free [NBP1-86162]")
Western Blot: ATP5D Antibody - BSA Free [NBP1-86162]
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla)
Applications for ATP5D Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATP5D
Alternate Names
ATP synthase subunit delta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit, F-ATPase delta subunit, mitochondrial ATP synthase complex delta-subunit precusor, mitochondrial ATP synthase, delta subunit
Gene Symbol
ATP5F1D
Additional ATP5D Products
Product Documents for ATP5D Antibody - BSA Free
Product Specific Notices for ATP5D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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