Ataxin-3 Antibody (HL1909) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25371
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (98%), Porcine (99%), Sheep (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1909 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Ataxin-3.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%), Chicken (89%), Xenopus (86%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Ataxin-3 Antibody (HL1909) - Azide and BSA Free (NBP3-25371) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Ataxin-3 Antibody (HL1909) - Azide and BSA Free
Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] -
Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] -
Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] -
Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Applications for Ataxin-3 Antibody (HL1909) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ataxin-3
Alternate Names
AT3, Ataxin3, ATXN3, JOS, MJD1, SCA3
Gene Symbol
ATXN3
Additional Ataxin-3 Products
Product Documents for Ataxin-3 Antibody (HL1909) - Azide and BSA Free
Product Specific Notices for Ataxin-3 Antibody (HL1909) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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