ASPP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40684

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ASPP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human Apoptosis-Stimulated Protein of p53, 1 using the numbering given in entry NP_056131.1 (GeneID 23368).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ASPP1 Antibody - BSA Free (NB110-40684) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ASPP1 Antibody - BSA Free

Western Blot: ASPP1 Antibody [NB110-40684]

Western Blot: ASPP1 Antibody [NB110-40684]

Western Blot: ASPP1 Antibody [NB110-40684] - Detection of Human ASPP1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.
Immunoprecipitation: ASPP1 Antibody [NB110-40684]

Immunoprecipitation: ASPP1 Antibody [NB110-40684]

Immunoprecipitation: ASPP1 Antibody [NB110-40684] - Detection of human ASPP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for IP at 6 ug per reaction. ASPP1 was also immunoprecipitated by rabbit anti-ASPP1 antibody NB110-40683. For blotting immunoprecipitated ASPP1, NB110-40684 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ASPP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASPP1

The ASPP family members ASPP1, ASPP2, act as potent activators of p53. A third member of the family, iASPP, has an inhibitory effect on apoptosis. The three members of ASPP family all bind p53 via their C-terminus, which contains a proline-rich region, four ankyrin repeats and an SH3 domain. Expression of ASPP1 and ASPP2 is frequently downregulated in human breast tumors expressing wild-type p53. ASPP1 may also play a role in vascular development.

Alternate Names

apoptosis-stimulating protein of p53, 1, ASPP1Protein phosphatase 1 regulatory subunit 13B, KIAA0771apoptosis-stimulating of p53 protein 1, p53BP2-like, p85, protein phosphatase 1, regulatory (inhibitor) subunit 13B

Entrez Gene IDs

23368 (Human)

Gene Symbol

PPP1R13B

UniProt

Q96KQ4 (Human)

Additional ASPP1 Products

Product Documents for ASPP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ASPP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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