ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49326
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQNHQT
Reactivity Notes
Mouse (85%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free (NBP2-49326) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
![Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]](https://resources.bio-techne.com/images/products/ASAH2-N-acylsphingosine-Amidohydrolase-2-Antibody-Western-Blot-NBP2-49326-img0003.jpg "Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]")
Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]
Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
![Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]](https://resources.bio-techne.com/images/products/ASAH2-N-acylsphingosine-Amidohydrolase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49326-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]")
Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]
Immunocytochemistry/Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.![Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]](https://resources.bio-techne.com/images/products/ASAH2-N-acylsphingosine-Amidohydrolase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49326-img0002.jpg "Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]")
Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]
Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Staining of human duodenum shows luminal membranous positivity in glandular cells.Applications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in intestinal brush border membranes, but is also expressed in the kidney, brain and liver. ASAH2 is involved in the metabolism of dietary sphingolipids and the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Product Specific Notices for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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