ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76360

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NBP1-76360-0.025mg
NBP1-76360
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Conjugate
Catalog #
Alexa Fluor 350
NBP1-76360AF350
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Alexa Fluor 405
NBP1-76360AF405
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Alexa Fluor 488
NBP1-76360AF488
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Alexa Fluor 532
NBP1-76360AF532
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Alexa Fluor 594
NBP1-76360AF594
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Alexa Fluor 647
NBP1-76360AF647
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Alexa Fluor 700
NBP1-76360AF700
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Alexa Fluor 750
NBP1-76360AF750
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Alexa Fluor Plus 405
NBP1-76360AFP405
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Alexa Fluor Plus 488
NBP1-76360AFP488
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Alexa Fluor Plus 555
NBP1-76360AFP555
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Alexa Fluor Plus 594
NBP1-76360AFP594
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Alexa Fluor Plus 647
NBP1-76360AFP647
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Alexa Fluor Plus 680
NBP1-76360AFP680
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Allophycocyanin
NBP1-76360APC
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Biotin
NBP1-76360B
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CoraFluor 1
NBP1-76360CL1
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DyLight 350
NBP1-76360UV
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DyLight 405
NBP1-76360V
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DyLight 488
NBP1-76360G
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DyLight 550
NBP1-76360R
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DyLight 594
NBP1-76360DL594
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DyLight 650
NBP1-76360C
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DyLight 680
NBP1-76360FR
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DyLight 755
NBP1-76360IR
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FITC
NBP1-76360F
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HRP
NBP1-76360H
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Janelia Fluor 525
NBP1-76360JF525
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Janelia Fluor 549
NBP1-76360JF549
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Janelia Fluor 646
NBP1-76360JF646
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Janelia Fluor 669
NBP1-76360JF669
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mFluor Violet 450 SE
NBP1-76360MFV450
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mFluor Violet 500 SE
NBP1-76360MFV500
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mFluor Violet 610 SE
NBP1-76360MFV610
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PE
NBP1-76360PE
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PerCP
NBP1-76360PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ASAH2/N-acylsphingosine Amidohydrolase-2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2. The immunogen is located within the last 50 amino acids of ASAH2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free

Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 AntibodyBSA Free [NBP1-76360]

Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 AntibodyBSA Free [NBP1-76360]

Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP1-76360] - ASAH2 Antibody 293 cell lysate with ASAHL antibody at (A) 1 and (B) 2 ug/ml.

Applications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-76360 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASAH2/N-acylsphingosine Amidohydrolase-2

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

Long Name

Neutral Ceramidase

Alternate Names

Nacylsphingosine Amidohydrolase2

Gene Symbol

ASAH2

UniProt

NP_063946 (Human)

Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products

Product Documents for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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