ARL3 Antibody - BSA Free

Western Blot: ARL3 Antibody [NBP1-88839]

Western Blot: ARL3 Antibody [NBP1-88839] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry-Paraffin: ARL3 Antibody [NBP1-88839]

Immunohistochemistry-Paraffin: ARL3 Antibody [NBP1-88839] - Staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.

Western Blot: ARL3 Antibody - BSA Free [NBP1-88839] -

Arl3-Y90C is a fast cycling GTPase.(A) 1% input (I) and eluates (E) from GST-PDE delta pulldowns using AD-293 lysates expressing 3XFLAG-tagged Arl3 mutants immunoblotted with anti-FLAG antibodies. (B) Arl3-Y90C-FLAG or Arl3-D129N-FLAG lysates were incubated with 10 mM ethylenediaminetetraacetic acid (EDTA) and/or 10 mM GTP, spiked with Mg2+, and precipitated with GST-PDE delta. Westerns immunoblotted for Arl3 with eluates shown on top, and 1% inputs shown below. Arl3-FLAG and endogenous Arl3 bands labeled. (C) Representative retinal cross-sections from Arl3-Y90C-FLAG- or Arl3-D129N-expressing rod photoreceptors stained with anti-FLAG antibodies (green) and counterstained with Hoechst (blue). Scale bars, 5 um. Nuclear position of electroporated rods represented as described in Figure 1.Figure 2—source data 1.Raw western blot images.Raw western blot images.GTP loading of wild-type Arl3-FLAG.Arl3-FLAG lysates were incubated with 10 mM EDTA and/or 10 mM GTP, spiked with Mg2+, and precipitated with GST-PDE delta. Westerns immunoblotted for Arl3 with eluates shown on top, and 1% inputs shown below. Arl3-FLAG and endogenous Arl3 bands labeled.Unlike Arl3-Q71L, Arl3-D67V does not form a ternary complex with RP2 and PDE delta.1% input (I) and eluates (E) from GST-PDE delta pulldowns using AD-293 lysates expressing 3XFLAG-tagged Arl3 mutants immunoblotted with anti-RP2 antibodies. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36598133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.