Aquaporin-7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30862
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Equine
Cited:
Mouse, Porcine, Bovine, Equine
Applications
Validated:
Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from amino acid region 2-50 of human Aquaporin-7 conjugated to blue carrier protein was used as the antigen.
Reactivity Notes
Equine reactivity reported in scientific literature (PMID: 29052325). Porcine reactivity reported in scientific literature (PMID: 28941027). Mouse reactivity reported in scientific literature (PMID: 31944177).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aquaporin-7 Antibody
Immunocytochemistry/ Immunofluorescence: Aquaporin-7 Antibody [NBP1-30862] -
Exogenous expression of EGFP and EGFP+AQP7 cRNA in bovine MII oocytes. (A) Immunofluorescence analysis of AQP7 expression in bovine oocytes of non-injected, EGFP cRNA-injected and EGFP+AQP7 cRNA-injected oocytes. Nuclei counterstained with DAPI are displayed in blue (a1–a3), EGFP expression is displayed in green (b1–b3) and AQP7 protein expression is displayed in red (c1–c3). Overlayed images are shown in (d1–d3). White asterisk indicates chromosomes; white arrowhead indicates polar body. Scale bar, 30 μm. (B) Summary data of the relative immunofluorescence intensity normalized to control. Data are presented in arbitrary units +/- SEM, n ≥ 20, a,b Different letters indicate statistically significant differences between treatments (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35203238), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: Aquaporin-7 Antibody [NBP1-30862] -
Effect of the exposure of IVM bovine oocytes to hyperosmotic CPA solutions on the levels of expression of AQP3 and AQP7. (A) Immunofluorescence analysis of AQP3 and AQP7 expression in bovine oocytes without treatment (a1 and a2, respectively) and in the presence of 9.5% Me2SO (b1 and b2, respectively), 8% EG (c1 and c2, respectively) or 0.5 M sucrose (d1 and d2, respectively). The concentrations of the two penetrating cryoprotectants, EG and Me2SO, were chosen to prepare solutions with similar osmolalities, as previously indicated [15]. AQP3 and AQP7 protein expression is displayed in green. Scale bar, 30 μm. (B) Summary data of the relative immunofluorescence intensity normalized to control. Data are presented in arbitrary units +/- SEM, n ≥ 25, a,b Different letters indicate statistically significant differences between treatments (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35203238), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Aquaporin-7 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reactivity reported in (PMID: 31944177)
Immunohistochemistry-Frozen
Reactivity reported in (PMID: 23628076)
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin-7
Alternate Names
AQP-7, AQP7L, AQP9MGC149556, AQPapaquaglyceroporin-7, Aquaglyceroporin-7, aquaporin 7, Aquaporin adipose, aquaporin-7, Aquaporin-7-like, MGC149555
Entrez Gene IDs
364 (Human)
Gene Symbol
AQP7
UniProt
Additional Aquaporin-7 Products
Product Documents for Aquaporin-7 Antibody
Product Specific Notices for Aquaporin-7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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