Type I membrane. Isoforms that contain the exon encoding for a furin-cleavage site are proteolytically processed, leading to a secreted receptor fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.

Immunocytochemistry/ Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

The identity of the AAV-BP is AAVR, the multiserotype AAV receptor. (A) Virus overlay assay of cell membrane fractions from different types of cells. Membrane proteins were extracted from various types of cells, and 100 μg of membrane proteins was used to perform a virus overlay assay with purified wild-type AAV2 particles (56). The arrow indicates a strong binding band at 150 kDa, designated AAV-BP. (B) Summary of the top five genes that correspond to the peptide sequences from the mass spectrometry analysis of the AAV-BP band. AAVR is also denoted KIAA0319L. (C) One hundred micrograms of PSA-purified HeLa S3 membrane proteins was separated on a 2-D gel and transferred onto a PVDF membrane for a virus overlay assay with rAAV2, followed by reprobing with anti-LDLR, anti-ApoER2, anti-ORP150, or anti-AAVR antibody. (D) One hundred micrograms of N- and O-deglycosylated crude HeLa S3 cell membrane proteins was separated by 2-D electrophoresis and underwent a virus overlay assay, followed by reprobing with a rabbit polyclonal antibody to integrin alpha5. Squares indicate an identical area of the membrane. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28679762), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Knockdown of LRP8 inhibited tumor growth in vivo. (a) The pictures of nude mice injected with Lv-sh-LRP8 and corresponding control and the tumors formed after 28-day feeding. (b–c) Tumor volumes and weights were calculated between the two groups. (d) Western blotting experiments detecting the expression of Wnt/ beta-catenin signaling components and LRP8 expression in subcutaneous tumors. **p < 0.01. Three independent trials in each experiment were needed, and the data were presented as mean +/- SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35246020), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

LRP8 enhanced the metastasis of NSCLC cells. (a) Transwell analysis was performed to compare the metastasis potential in the LRP8 knockdown group. (b) H1975 cells migration and invasion capability were detected by transwell assays. The expression of proteins related to metastasis in H1299 and H460 cells (c) and H1975 cells (d) were detected by Western blotting. **p < 0.01. At least three replicate experiments were performed, and the final results were presented as mean +/- SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35246020), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

LRP8 motivated NSCLC cells development and metastasis via the Wnt/ beta-catenin signaling pathway. (a) Expression of Wnt/ beta-catenin signaling components after silencing LRP8 as detected by Western blotting assays. (b) Expression of Wnt/ beta-catenin signaling-related factors in overexpression LRP8 group of H1975 cells. CCK-8 assay (c) and colony formation analysis (d) were carried out to evaluate the proliferation abilities of H1299 and H460 cells transfected with LRP8 siRNA or negative vector or LiCl and LRP8 siRNA. (e) Invasion and migration of H1299 and H460 cells after LRP8 downregulation and LiCl addition as detected by Transwell assay. (f) Western blotting analysis for E-cadherin, Vimentin, and N-cadherin to detect the effect of LiCl in LRP8 knockdown. (g) Western blotting assays were performed to elaborate the role of LiCl in Wnt/ beta-catenin signaling-related factors induced by LRP8 silencing. **p < 0.01. Each experiment was repeated in three independent trials, and mean +/- SD was used to describe the results. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35246020), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

LRP8 is related to the poor prognosis of NSCLC patients. (a–c) Expression of LRP8 in NSCLC tissues as analyzed by TIMER and StarBase3.0 databases. (d) Western blotting assays to detect the expression of LRP8 in seven NSCLC cell lines and the normal bronchial epithelioid cells. (e) Immunohistochemistry staining of two representative cases showing the expression and location of LRP8 in NSCLC tissues. (f) Four-grid table showing the statistical difference of LRP8 level between tumor tissues and normal adjacent tissues. (g) Kaplan–Meier curve based on LRP8 expression in 60 NSCLC patients (log-rank test, p < 0.05). n.s, no significant difference, *p < 0.05, **p < 0.01. At least three independent biological experiments were repeated, and the data were presented as mean +/- SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35246020), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

LRP8 promoted NSCLC proliferation in vitro. Western blotting experiments were conducted to validate the transfection efficiency of LRP8 siRNA in H460 and H1299 (a) and LRP8 overexpression plasmid in H1975 (d). CCK-8 assay was used to evaluate the proliferation ability of H460 and H1299 cells transfected with LRP8 siRNAs (b) and H1975 cells with LRP8 plasmid (e). (c) Colony formation analysis showing differences in H1299 and H460 cell proliferation among the three groups. (f) H1975 cell viability was measured using colony formation analysis. **p < 0.01. All experiments were performed independently at least three times, and the results were presented as mean +/- SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35246020), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

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Applications for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:500

SDS-Page

reported in scientific literature (PMID 27810638)

Western Blot

2 ug/ml

Application Notes

A band is seen at ~106 kDa representing the membrane form of ApoER2 in Western Blot. A larger ~130 kDa band may also be seen, representing the glycosylated form of ApoER2.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E R2/ApoE R2

ApoER2 (apolipoprotein E receptor 2 or LDL receptor related protein 8) is a member of LDL receptor (LDLR) family and it acts as a cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. ApoER2 facilitates extracellular RELN signal transduction to intracellular signaling processes, mainly through binding with DAB1 on its cytoplasmic tail. RELN acts via both VLDLR and ApoER2 to regulate tyrosine phosphorylation mediated DAB1 activation and microtubule function in neuronal cells. ApoER2 possess higher affinity for RELN compared to VLDLR and is therefore, a key player in Reelin pathway that governs forebrain's neuronal layering during prenatal brain development. ApoER2 associates with ER resident receptor-associated protein (RAP) as well as with dimers of beta 2-glycoprotein I. In the vasculature, ApoER2 has been suggested to implicate in suppressing platelet aggregation. Moreover, ApoER2 is highly expressed in epididymis (initial segment), where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), which are required for sperm maturation. ApoER2 also function as an endocytic receptor and defective ApoER2 have been linked to myocardial infarction type 1 (MCI1).

Long Name

Apolipoprotein E Receptor 2

Alternate Names

ApoER2, Lr8b, LRP-8, LRP8

Gene Symbol

LRP8

Additional Apolipoprotein E R2/ApoE R2 Products

Product Documents for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2216

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Apolipoprotein E R2/ApoE R2 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody - BSA Free [NB100-2216] -

Applications for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

SDS-Page

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Apolipoprotein E R2/ApoE R2

Long Name

Alternate Names

Gene Symbol

Additional Apolipoprotein E R2/ApoE R2 Products

Product Documents for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free