53BP1 Antibody [DyLight 650]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [DyLight 650] [NB100-305C] -

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [DyLight 650] [NB100-305C] - Dual-color dSTORM acquisition workflow with fluorescence nanodiamonds (FNDs). (a) Representative dSTORM images of MCF10A cells upon targeting of 53BP1 (red) & DYNLL1 (green), stained with AlexaFluor647 & CF568, respectively. Shown are (from left to right) the single-color 53BP1 & DYNLL1 images & the dual-color dSTORM image. (b) FNDs spots positions obtained from red & green STORM channels. (c) Zoomed region of the highlighted area (white dashed box) in (b). Upper panel the localization events (each colored pixel represents one localized single-molecule event) recorded during the acquisition. Representative FNDs are indicated by the arrows. In the lower panel, the recorded events were transformed into Gaussian PSFs of fixed width & segmented (see Materials & Methods & Supplementary Protocol for details) to highlight the drift correction (Scale bar ROI: 1 μm) (d) Representative images of dual-color dSTORM acquisition. The fluorophore blinking in timelapse imaging (15,000 frames) for each channel (53BP1-AlexaFluor647 & DYNLL1-CF568) is shown. The bright spots in the image correspond to the fluorescence emission of individual fluorophores. Most of the fluorescent labels are switched off such that the fluorescent molecules are well separated. FNDs show no blinking properties, indicating high photostability in both channels. (e,f) Comparison of the photon emission from fluorescent nanodiamonds (FND, 40 nm; black), Alexa Fluor 647 dye (red) & CF568 dye (green) over 2000 acquired frames using 561 nm & 647 nm laser excitations, measured in the highlighted regions (white dashed box). (g) Fluorescence decrease of the 40 nm FNDs (black) & the AlexaFluor647 (red), over 15,000 frames of image. Scale bar: 3 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35215014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.