TdT In Situ Apoptosis Detection Kit - Core Reagents
R&D Systems, part of Bio-Techne | Catalog # 4810-30-CK
In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Discontinued Product
4810-30-CK has been discontinued.
View all TUNEL Assays products.
Key Product Details
Features
Provides a streptavidin-HRP conjugate without an HRP-substrate or a counterstain.
Key Benefits
- TdT based in situ labeling.
- Cation optimization for high signal and low background.
- Includes exclusive Cytonin permeabilization reagent.
- Includes TACS-Nuclease control reagents.
- Light microscopy or Fluorescence microscopy options.
Species
Multi-Species
Product Summary for TdT In Situ Apoptosis Detection Kit - Core Reagents
Why Use the TdT In Situ Apoptosis Detection Kit - Core Reagents?
TACS 2 TdT Core kit utilize unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher's choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.
Product Specifications
- In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Kit Contents for TdT In Situ Apoptosis Detection Kit - Core Reagents
- Streptavidin-HRP
- TACS 2 TdT Labeling Buffer
- TACS 2 TdT Stop Buffer
- TACS 2 TdT dNTP
- TdT Enzyme
- 50x Cobalt Cation
- 50x Magnesium Cation
- 50x Manganese Cation
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for TdT In Situ Apoptosis Detection Kit - Core Reagents
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