Calcein AM Cell Viability Assay

Name: Calcein AM Cell Viability Assay
Brand: R&D Systems
SKU: 4892-010-K
Price: 238 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 4892-010-K

Citations (13)

Product Datasheet / COA / SDS

Key Product Details

Features:	The Calcein AM Cell Viability Assay provides a simple, rapid and accurate method to measure cell viability and/or cytotoxicity.
Key Benefits:	Suitable for proliferating and non-proliferating cells. Ideal for both suspension and adherent cells. Non-radioactive assay. Rapid (no solubilization step as in a conventional MTT assay). Ideal for high-throughput experiments. Better retention and brightness compared to other fluorescent compounds

Discontinued Product

4892-010-K has been discontinued. View all Cell Viability/Proliferation Assays products.

Setting the standard in quality research reagents

Technical Data
Citations (13) / Reviews

Summary for Calcein AM Cell Viability Assay

Why Use the Calcein AM Cell Viability Assay?

Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours. Calcein AM is useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.

Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.

What is Calcein AM?

Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

Kit Contents for Calcein AM Cell Viability Assay

Calcein AM
10X Calcein AM DW Buffer

Preparation & Storage

Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Citations for Calcein AM Cell Viability Assay (13)

Showing 1 - 10 of 13 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Calcein AM Cell Viability Assay include:

Species: Mouse
Sample Types: Whole Cells

C Ngwa et al. (2021), Phosphorylation of Microglial IRF5 and IRF4 by IRAK4 Regulates Inflammatory Responses to Ischemia Cells, 10(2).
PMID: 33573200
Species: Human
Sample Types: Whole Cells

K Mörs et al. (2021), Anti-inflammatory Effects of Alcohol Are Associated with JNK-STAT3 Downregulation in an In Vitro Inflammation Model in HepG2 Cells Disease Markers, 2021(0):6622701.
PMID: 33791043
Species: Human
Sample Types: Whole Cells

S Kim et al. (2021), Transcorneal delivery of topically applied silver nanoparticles does not delay epithelial wound healing NanoImpact, 24(0):100352.
PMID: 35559825
Species: Human
Sample Types: Whole Cells

JA Hörauf et al. (2020), Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro Int J Mol Sci, 21(9).
PMID: 32366053
Species: E. Coli
Sample Types: Whole Cells

Y Liu et al. (2020), A hidden risk: Survival and resuscitation of Escherichia coli O157:H7 in the viable but nonculturable state after boiling or microwaving Water Res., 183(0):116102.
PMID: 32745672
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Lee et al. (2019), Effective breast cancer combination therapy targeting BACH1 and mitochondrial metabolism Nature, 0(0).
PMID: 30842661
Species: Human
Sample Types: Whole Cells

S Zaman et al. (2019), Combinatorial therapy of Zinc metallochaperones with mutant p53 reactivation and diminished copper binding Mol. Cancer Ther., 0(0).
PMID: 31196889
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Nguemgo Ko et al. (2018), Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells PLoS ONE, 13(6):e0198508.
PMID: 29864155
Species: Human
Sample Types: Whole Cells

MM Olcina et al. (2018), Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer Cell Rep, 25(13):3721-3732.e6.
PMID: 30590044
Species: Human
Sample Types: Whole Cells

H Zhang et al. (2017), Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke Aging Dis, 8(2):203-214.
PMID: 28400986
Species: Human
Sample Types: Whole Cells

Jonathan D Diedrich et al. (2016), Bone marrow adipocytes promote the Warburg phenotype in metastatic prostate tumors via HIF-1? activation. Oncotarget, 0(0):1949-2553.
PMID: 27588494
Swain L et al. (2014), Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions. J Leukoc Biol, 96(3):365-75.
PMID: 24626957
Species: Mouse
Sample Types: Whole Cells

Giachin et al. (2014), Prion protein interaction with soil humic substances: environmental implications. PLoS ONE, 9(6):e100016.
PMID: 24937266

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