Biotin-NAD+

Name: Biotin-NAD+
Brand: Tocris Bioscience
SKU: 6573

Tocris Bioscience, part of Bio-Techne | Catalog # 6573

Citations (10)

COA / SDS

Biotinylated-NAD+; substrate for ADP-ribosylation

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Technical Data
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Product Description
Product Specifications
Solubility
Calculators
Background References
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Specific Notices

Key Product Details

Description

Biotinylated-NAD+; substrate for ADP-ribosylation

View all Biochemicals and Molecular Biology Compounds Activity Assays »

Product Description

Biotin-NAD+ is a provides a convenient non-isotopic alternative to radiolabeled NAD⁺ for determination of IC₅₀ values for candidate PARP inhibitors and studies requiring this substrate. Biotinylated-NAD⁺ allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system. Acts as a substrate for ADP-ribosylation. Can be used to label and purify biotinyl-ADP ribosylated proteins.

This product is a replacement for R&D Systems product 4670-500-01.

131 μg is supplied as 500 μl of a 0.25 mM solution in water.

Product Specifications

Molecular Weight

1045.95

Formula

C₃₉H₅₇N₁₁O₁₇P₂S

Storage

Store at -80°C

Chemical Name

β-Nicotinamide-N⁶-[2-[[6-[biotinyl]amino]hexyl]amino]-2-oxoethyl]adenine dinucleotide

CAS Number

146385-37-7

InChI Key

MIIADCCJYSONEI-RNWYXDGLSA-N

SMILES

[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCCNC(CNC3=C(N=CN4[C@@H]5O[C@@H]([C@H]([C@H]5O)O)COP(O)(OP(OC[C@H]6O[C@@H]([N+]7=CC=CC(C(N)=O)=C7)[C@H](O)[C@@H]6O)([O-])=O)=O)C4=NC=N3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Soluble in water (supplied pre-dissolved at a concentration of 0.25mM)

Calculators

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Background References

References are publications that support the biological activity of the product.

Yang Antitumor activity of a pyrrole-imidazole polyamide. Proc.Natl.Acad.Sci.USA 2013 PMID: 23319609
Yang Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9 Mol. Cell 2017 PMID: 28525742
Schuster The Hsp90 machinery facilitates the transport of diphtheria toxin into human cells. Sci.Rep. 2017 PMID: 28377614
Zhang Purification of a nitric oxide-stimulated ADP-ribosylated protein using biotinylated beta-nicotinamide adenine dinucleotide. Biochemistry 1993 PMID: 8443164