ATPase Activity Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59733

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP2-59733 has been discontinued. View all ATPase Activity products.

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Mammal

Specificity

Rapid assessment of ATPase activity in biological samples or recombinant ATPase preparations.

Description

This kit provides a quick and easy method for monitoring ATPase activity in various samples. This ATPase assay hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated. The assay is designed to measure ATPase activity in biological samples with detection sensitivity ~5 mU/l. Detection method: Absorbance at 650 nm.

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab234055)

Scientific Data Images for ATPase Activity Activity Assay Kit (Colorimetric)

ATPase Activity Activity Assay Kit (Colorimetric) [NBP2-59733]
ATPase Activity Activity Assay Kit (Colorimetric) [NBP2-59733]

Kit Contents for ATPase Activity Activity Assay Kit (Colorimetric)

  • ATPase Assay Buffer
  • ATPase Developer
  • ATPase Positive Control
  • ATPase Substrate
  • Phosphate Standard (10 mM)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: ATPase Activity

ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ ATPase, H+/K+ ATPase and Ca2+ ATPase. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+ ATPase deficiency increases anxiety-related behavior, while Ca2+ ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases.

Product Documents for ATPase Activity Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATPase Activity Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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