APO-DIRECT (TUNEL) Apoptosis Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31159

Novus Biologicals, part of Bio-Techne
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NBP2-31159
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Key Product Details

Species

Multi-Species

Applications

Flow Cytometry

Kit Type

Apoptosis Kit

Product Summary for APO-DIRECT (TUNEL) Apoptosis Kit

The APO-DIRECT™ kit is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks (FITC) along with total cellular DNA (Propidium Iodide) to detect apoptotic cells by flow cytometry or laser scanning cytometry. The kit contains all necessary reagents including positive and negative controls. APO-DIRECT(TM) is a trademark of Phoenix Flow Systems, San Diego, CA.

Product Specifications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27459668)

Kit Contents for APO-DIRECT (TUNEL) Apoptosis Kit

  • FITC dUTP
  • Negative Control Cells
  • Positive Control Cells
  • Propidium Iodide/RNase DNA staining solution
  • Reaction Buffer
  • Rinsing Buffer
  • TdT Enzyme
  • Wash Buffer

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: APO-DIRECT (TUNEL)

Apoptosis is programmed cell death. It is believed to take place in most eukaryotic cells. It is a distinct event that triggers characteristic morphological and biological changes in the cellular life cycle. It is common during embryogenisis, normal tissue and organ involution, cytotoxic immunological reactions and occurs naturally at the end of the life span of differentiated cells. It can also be induced in cells by the application of several different agents including physiological activators, heat shock, bacterial toxins, oncogenes, chemotherapeutic drugs, ultraviolet and gamma radiation. When apoptosis occurs, the nucleus and cytoplasm of the cell often fragment into membrane-bound apoptotic bodies that are then phagocytized by neighboring cells. Alternatively, during necrosis, cell death occurs by direct injury to cells resulting in cellular lysing and release of cytoplasmic components into the surrounding environment often inducing an inflammatory response in the tissue. A landmark of cellular self- destruction by apoptosis is the activation of nucleases that degrade the higher order chromatin structure of the DNA into fragments of 50 to 300 kilobase pairs and subsequently into smaller DNA pieces of about 200 base pairs in length. Numerous reviews of the events accompanying apoptosis are available and several well-researched model systems have been described.

Alternate Names

APO DIRECT, APO-DIRECT, TUNEL, TUNEL assay

Additional APO-DIRECT (TUNEL) Products

Product Documents for APO-DIRECT (TUNEL) Apoptosis Kit

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APO-DIRECT (TUNEL) Apoptosis Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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