Webinar: Enriching High Titer IgG-producing Clones Using Cold-Capture and the Namocell Pala Single Cell Dispenser

Cloning of Chinese hamster ovary (CHO) cells expressing recombinant antibodies is a crucial step in the development of monoclonal antibody (mAb) therapeutics. It is important to identify clones that produce high yield. However, the heterogeneity of CHO cells can give rise to varied IgG production among individual cells. Thus, the process of finding high titer clones typically involves screening of large numbers of clones, making it laborious and time-consuming.

Cold-capture is a technique that allows labelling of antibody producing cells with a fluorescent reagent that binds to the antibody molecules when they are transiently present on the cell membrane before release from secretory vesicles. This labelling could enable cell sorting based on the level of fluorescence signal to select higher productivity cells. In this study, the featured speaker sought to test the ability to enrich high titer IgG CHO cell clones by cold-capture, followed by isolation of single cells with the brightest fluorescent signal using the Namocell Pala Single Cell Dispenser — a fully automated dispenser that enables a fast, easy fluorescence and light scatter-based single cell isolation. The speaker will show that their method allowed the identification of clones with an average 3x higher IgG production from the cold-capture labelled cells compared to non-cold-captured cells.

These results suggest that the combination of cold-capture and single cell isolation could be an efficient and economical solution for improving cell line development in recombinant antibody production.

Watch now to learn more about the potential of cold-capture-facilitated single cell isolation and cloning to improve the efficiency in identifying high yield clones.