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Multiplex to the Max with Single-Cell Westerns

Want to measure expression of multiple proteins in each single cell in your sample? Milo can multiplex 12+ targets simultaneously and can achieve multiplexing levels that match or exceed the most powerful multiplexed flow cytometers.


Single-Cell Westerns with Milo™ allow you to unlock the single-cell proteome and measure changes in protein expression heterogeneity that aren’t possible with other techniques. Whether you need to conserve limited samples, compare relative abundance of protein isoforms or a phosphorylated form to its total abundance, assess the expression of proteins with a similar molecular weight, or just want to confirm a result with a different antibody, Milo is a powerful solution that lets you do all of this at the single-cell level. Make the most of your research with multiplexing on Milo.

Multiplex on Milo using one or more of the following strategies:

  • Size-based multiplexing: Use the SDS-PAGE separation to resolve proteins that differ in molecular weight
  • Spectral multiplexing: Use antibodies labeled with distinct fluorophores and image them in different spectral channels on your microarray scanner.
  • Stripping & re-probing: Since your sample is covalently bound into the gel after the SDS-PAGE separation and UV light exposure, antibodies can be stripped off with minimal sample losses. This enables multiple rounds of stripping & reprobing on Milo – something that isn’t typically possible with conventional Westerns which rely on weaker electrostatic or hydrophobic interactions to immobilize the sample on a membrane.

Want to learn how to multiplex with Milo?

Our technical note will walk you through multiplexing with Milo and provide you with evidence of and guidance for efficient stripping to achieve multiplexing levels that match or exceed those of the most powerful multiplexed flow cytometers.


Why Single-Cell Westerns?

Learn how Single-Cell Westerns on Milo can take you beyond traditional Western blotting and flow cytometry. Explore the benefits.