Nicotinamide

Name: Nicotinamide
Brand: Tocris Bioscience
SKU: 4106
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4106

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Citations (57)

COA / SDS

PARP-1 inhibitor

Key Product Details

Description:	PARP-1 inhibitor
Chemical Name:	Pyridine-3-carboxamide
Purity:	≥99% (HPLC)
Molecular Weight:	122.12

View Full Technical Details for Nicotinamide

Technical Data
Citations / Reviews (1)

Biological Activity for Nicotinamide

Nicotinamide is an anti-inflammatory agent. Inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. NAD⁺ precursor. Promotes differentiation of mesenchymal stem cells to insulin producing cells when used in combination with growth factors and high glucose concentration. Nicotinamide also acts as a SIRT1 inhibitor and promotes the expansion of hematopoietic progenitor cells. Nicotinamide can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells.

Nicotinamide synthesized to Ancillary Material Grade also available.

For more information about how Nicotinamide may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Compound Libraries for Nicotinamide

Nicotinamide is also offered as part of the Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Scientific Data Examples for Nicotinamide

	Application of Nicotinamide in Culture and Characterization of iPSC-derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were cultured using Nicotinamide, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (p38 MAPK inhibitor) (Catalog # 1264), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; green), a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).
	Application of Nicotinamide in Culture and Characterization of Adult Stem Cell-derived Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes Nicotinamide, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), SB 202190 (Catalog # 1264), along with the other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
	Application of Nicotinamide in Culture and Characterization of Adult Stem Cell-derived Liver Organoids. Adult stem cell-derived liver organoids were cultured using Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-0 (Catalog # 2939), Forskolin (Catalog # 1099), N-Acetylcysteine (Catalog # 7874), along with the other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Nicotinamide, HEPES (Catalog # 3173), N-Acetylcysteine (Catalog # 7874), A 83-0 (Catalog # 2939), along with the other reagents used in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).
	Application of Nicotinamide in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids. Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and cultured for 30 days in intestinal organoid culture medium, which includes Nicotinamide, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), along with the other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification.

Technical Data for Nicotinamide

M. Wt	122.12
Formula	C₆H₆N₂O
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	98-92-0
PubChem ID	936
InChi Key	DFPAKSUCGFBDDF-UHFFFAOYSA-N
Smiles	NC(C1=CC=CN=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nicotinamide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	12.21	100

Preparing Stock Solutions for Nicotinamide

The following data is based on the product molecular weight 122.12.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	8.19 mL	40.94 mL	81.89 mL
5 mM	1.64 mL	8.19 mL	16.38 mL
10 mM	0.82 mL	4.09 mL	8.19 mL
50 mM	0.16 mL	0.82 mL	1.64 mL

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References for Nicotinamide

References are publications that support the biological activity of the product.

Peled Nicotinamide, a SIRT1 inhibitor, inhibits differentiation and facilitates expansion of hematopoietic progenitor cells with enhanced bone marrow homing and engraftment. Exp.Hematol. 2012 PMID: 22198152
Jung Isolation and in vitro expansion of human colonic stem cells. Nat.Med. 2011 PMID: 21892181
Boj Organoid models of human and mouse ductal pancreatic cancer. Cell 2015 PMID: 25557080
Bartfeld In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 2015 PMID: 25307862
Sato SnapShot: Growing organoids from stem cells. Cell 2015 PMID: 26091044
Czubak A modified method of Ins producing cells' generation from bone marrow-derived mesenchymal stem cells. J.Diabetes Res. 2014 PMID: 25405207
Cimadamore Nicotinamide rescues human embryonic stem cell-derived neuroectoderm from parthanatic cell death. Stem Cells. 2009 PMID: 19544437
Suzuki Protective effect of nicotinamide against poly(ADP-ribose) polymerase-1-mediated astrocyte death depends on its transporter-mediated uptake. Life.Sci. 2010 PMID: 20188745