DAPI
Tocris Bioscience, part of Bio-Techne | Catalog # 5748
Key Product Details
| Description: | Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH |
| Alternative Names: | DAPI Dye |
| Chemical Name: | 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 350.25 |
Biological Activity for DAPI
Key information: DAPI is a widely used blue-fluorescent DNA stain / dye. Membrane impermeant to live cells. For nuclear counterstain, fixed and live-cell staining (at high concentration).
Used for: nuclear counterstain, fixed and live-cell staining (at high concentration), DNA visualization, chromosome staining, DNA staining in agarose gels, apoptosis analysis, mouse embryos or fetal organs visualization.
Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), and ISH.
Properties and Photophysical Data: DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 350 nm and 470 nm, respectively.
Optical Data for DAPI
| Emission Color | Blue |
|---|---|
| λabs | 350 nm |
| λem | 470 nm |
| Closest Laser line | 405 nm |
| Application | Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC) |
|
|
DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm. |
|
DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm. |
|
RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white). |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer