SB 202190

Name: SB 202190
Brand: Tocris Bioscience
SKU: 1264
Rating: 3 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1264

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Citations (319)

COA / SDS

Potent, selective inhibitor of p38 MAPK

Key Product Details

Description:	Potent, selective inhibitor of p38 MAPK
Chemical Name:	4-[4-(4-Fluorophenyl)-5-(4-pyridinyl)-1H-imidazol-2-yl]phenol
Purity:	≥99% (HPLC)
Molecular Weight:	331.35

View Full Technical Details for SB 202190

Technical Data
Citations / Reviews (1)

Biological Activity for SB 202190

SB 202190 is a highly selective, potent and cell-permeable inhibitor of p38 MAP kinase. Binds within the ATP pocket of the active kinase (K_d = 38 nM, as measured in recombinant human p38), and selectively inhibits the p38α and β isoforms (IC₅₀ = 50 and 100 nM at SAPK2a/p38 and SAPK2b/p38β2 respectively). Promotes stability of naive human pluripotent stem cells in culture. Inhibits Yamanaka factor reprogramming of human fibroblasts to iPSCs. SB 202190 improves the self-renewal ability of neuronal stem cells from NPC1-deficient mice.

Licensing Information

Sold with the permission of GlaxoSmithKline

Compound Libraries for SB 202190

SB 202190 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Scientific Data Examples for SB 202190

	Application of SB 202190 in Culture and Characterization of iPSC-derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were cultured using SB 202190, Y-27632 dihydrochloride (Catalog # 1254), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; green), a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).
	Application of SB 202190 in Culture and Characterization of Adult Stem Cell-derived Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes SB 202190, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with the other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
	Application of SB 202190 in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids. Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and cultured for 30 days in intestinal organoid culture medium, which includes SB 202190, Y-27632 dihydrochloride (Catalog # 1254), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106),, along with the other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification.

Technical Data for SB 202190

M. Wt	331.35
Formula	C₂₀H₁₄N₃OF
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	152121-30-7
PubChem ID	16759148
InChi Key	YKRNPHOBDOUQTG-UHFFFAOYSA-N
Smiles	OC1=CC=C(C=C1)C1=NC(=C(N1)C1=CC=NC=C1)C1=CC=C(F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SB 202190

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	33.13	100

Preparing Stock Solutions for SB 202190

The following data is based on the product molecular weight 331.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.02 mL	15.09 mL	30.18 mL
5 mM	0.60 mL	3.02 mL	6.04 mL
10 mM	0.30 mL	1.51 mL	3.02 mL
50 mM	0.06 mL	0.30 mL	0.60 mL

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References for SB 202190

References are publications that support the biological activity of the product.

Sato Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology 2011 PMID: 21889923
Yang NPC1 gene deficiency leads to lack of neural stem cell self-renewal and abnormal differentiation through activation of p38 mitogen-activated protein kinase signaling. Stem Cells 2006 PMID: 16099992
Karthaus Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell 2014 PMID: 25201529
Neganova A critical role for p38MAPK signalling pathway during reprogramming of human fibroblasts to iPSCs. Sci.Rep. 2017 PMID: 28155868
Sato SnapShot: Growing Organoids from Stem Cells. Cell 2015 PMID: 26091044
Bartfeld In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 2015 PMID: 25307862
Gafni Derivation of novel human ground state naive pluripotent stem cells. Nature 2013 PMID: 24172903
Nemoto Induction of apoptosis by SB 202190 through inhibition of p38β mitogen-activated protein kinase. J.Biol.Chem. 1998 PMID: 9632706
Jiang Characterization of the structure and function of a new mitogen-activated protein kinase (p38β). J.Biol.Chem. 1996 PMID: 8663524
Frantz The activation of p38 mitogen-activated protein kinase determines the efficiency of ATP competition for pyridinylimidazole inhibitor binding. Biochemistry 1998 PMID: 9753474
Davies Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 2000 PMID: 10998351