Compound E
Tocris Bioscience, part of Bio-Techne | Catalog # 6476
γ-secretase inhibitor; induces neuronal differentiation
Key Product Details
Description
γ-secretase inhibitor; induces neuronal differentiation
Alternative Names
γ-Secretase Inhibitor XXI
Product Description
Compound E is a γ-secretase and notch pathway inhibitor. Compound E enhances growth inhibition, differentiation and migration of neuroblastoma cells when used in a combination with DAPT (Cat. No. 2634) and 13-cis RA. In combination with hLIF, CHIR 99021 (Cat. No. 4423) and SB 431542 (Cat. No. 1614), Compound E accelerates the induction of a homogeneous, self-renewing primitive neuroepithelium population from hESCs. Compound E is active in vitro and in vivo. Used in protocol to generate pancreatic beta cells from hPSCs (see below).For more information about how Compound E may be used, see our protocol: Generation of β cells from hPSCs
This product is Bio-Techne's ancillary Material Grade version of Compound E (Cat. No. 6476). Suitable for use in research and further manufacturing of cell therapies. Find out more about our Ancillary Material Grade product range.
Product Specifications
Molecular Weight
490.50
Formula
C27H24F2N4O3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
N-[(1S)-2-[[(3S)-2,3-Dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepin-3-yl]amino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide
CAS Number
209986-17-4
PubChem ID
11306390
InChI Key
JNGZXGGOCLZBFB-IVCQMTBJSA-N
SMILES
C[C@@H](C(N[C@H]1N=C(C2=CC=CC=C2N(C1=O)C)C3=CC=CC=C3)=O)NC(CC4=CC(F)=CC(F)=C4)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 49.05 | 100 |
Preparing Stock Solutions
for Compound E
The following data is based on the product molecular weight 490.50
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.04 mL | 10.19 mL | 20.39 mL |
| 5 mM | 0.41 mL | 2.04 mL | 4.08 mL |
| 10 mM | 0.20 mL | 1.02 mL | 2.04 mL |
| 50 mM | 0.04 mL | 0.20 mL | 0.41 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Ferrari-Toninelli Targeting Notch pathway induces growth inhibition and differentiation of neuroblastoma cells. Neuro.Oncol. 2010 PMID: 20716592
- Beher Pharmacological knock-down of the presenilin 1 heterodimer by a novel gamma -secretase inhibitor: implications for presenilin biology. J.Biol.Chem. 2001 PMID: 11574530
- Li Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. Proc.Natl.Acad.Sci.USA 2011 PMID: 21525408
Product Specific Notices for Compound E
For research use only
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