DAPT

Name: DAPT
Brand: Tocris Bioscience
SKU: 2634
Rating: 5 (6 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2634

(6)

Citations (468)

COA / SDS

γ-secretase inhibitor; induces neuronal differentiation; blocks Notch signaling

Key Product Details

Description:	γ-secretase inhibitor; induces neuronal differentiation; blocks Notch signaling
Chemical Name:	(2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester
Purity:	≥99% (HPLC)
Molecular Weight:	432.46

View Full Technical Details for DAPT

Technical Data
Citations / Reviews (6)

Biological Activity for DAPT

DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC₅₀ values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical and dopaminergic neurons from hPSCs (see our protocols below). Used in protocols to generate myoblasts from human human iPSCs. DAPT also promotes the formation of cone photoreceptors in retinal organoids.

For more information about how DAPT may be used, see our protocols: Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts, Generating Midbrain Dopaminergic Neurons from hPSCs.

DAPT synthesized to Ancillary Material Grade also available.

Compound Libraries for DAPT

DAPT is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Scientific Data Examples for DAPT

	Dopaminergic Neurons Derived from hiPSCs using DAPT. Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT and Purmorphamine (Catalog # 4551, Tocris). Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Technical Data for DAPT

M. Wt	432.46
Formula	C₂₃H₂₆F₂N₂O₄
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	208255-80-5
PubChem ID	5311272
InChi Key	DWJXYEABWRJFSP-XOBRGWDASA-N
Smiles	CC(C)(C)OC([C@H]([C@]2=CC=CC=C2)NC([C@H](C)NC(CC1=CC(F)=CC(F)=C1)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DAPT

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	43.24	100

Preparing Stock Solutions for DAPT

The following data is based on the product molecular weight 432.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.31 mL	11.56 mL	23.12 mL
5 mM	0.46 mL	2.31 mL	4.62 mL
10 mM	0.23 mL	1.16 mL	2.31 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

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References for DAPT

References are publications that support the biological activity of the product.

Xiang Long-term functional maintenance of primary human hepatocytes in vitro. Science 2019 PMID: 31023926
Zerti Developing a simple method to enhance the generation of cone and rod photoreceptors in pluripotent stem cell-derived retinal organoids. Stem Cells 2020 PMID: 31670434
Qi Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells. Nat.Biotechnol. 2017 PMID: 28112759
Sato Snapshot: growing organoids from stem cells. Cell 2015 PMID: 26091044
Crawford and Roelink The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev.Dyn. 2007 PMID: 17295317
De Smedt Different thresholds of notch signaling bias human precursor cells towards B-, NK-, monocytic/dendritic, or T-cell lineage in thymus microenvironment. Blood 2005 PMID: 16030192
Dovey Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J.Neurochem. 2001 PMID: 11145990