SB 431542

Product Description

SB 431542 is a potent and selective inhibitor of the transforming growth factor-β (TGF-β) type I receptor/ALK5 (IC₅₀ = 94 nM), and its relatives ALK4 and ALK7. Suppresses TGF-β-induced proliferation of human osteosarcoma cells. Replaces SOX2 in reprogramming of fibroblasts into iPSCs. Stimulates proliferation, differentiation and sheet formation of ESC-derived endothelial cells. Inhibits TGF-β-induced EMT, migration, invasion and VEGF secretion in several human cancer cell lines. Also used in a protocol to generate brain organoids from human iPSCs. Can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells, or vascular organoids. Also used in a protocol to generate cortical neurons from hiPSCs or dopaminergic neurons from hPSCs (see our protocol below).

SB 431542 synthesized to cGMP guidelines also available.

For more information about how SB 431542 may be used, see our protocols: 3D Culture of Lung Alveolar Cells, Differentiation of Hematopoietic Progenitors from hPSCs, Generating Midbrain Dopaminergic Neurons from hPSCs, Generating Vascular Organoids, Accelerated Induction of Cortical Neurons from hiPSCs.

Solubility

Preparing Stock Solutions for SB 431542

The following data is based on the product molecular weight 384.39

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.60 mL	13.01 mL	26.02 mL
5 mM	0.52 mL	2.60 mL	5.20 mL
10 mM	0.26 mL	1.30 mL	2.60 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Schafer An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 2023 PMID: 37172564
• Halder A specific inhibitor of TGF-beta receptor kinase, SB-431542, as a potent antitumor agent for human cancers. Neoplasia 2005 PMID: 15967103
• Watabe TGF-beta receptor kinase inhibitor enhances growth and integrity of embryonic stem cell-derived endothelial cells. J.Cell Biol. 2003 PMID: 14676305
• Matsuyama SB-431542 and Gle. inhibit transforming growth factor-β-induced proliferation of human osteosarcoma cells. Cancer Res. 2003 PMID: 14633705
• Laping Inhibition of transforming growth factor (TGF)-β1-induced extracellular matrix with a novel inhibitor of the TGF-β type I receptor kinase activity: SB-431542. Mol.Pharmacol. 2002 PMID: 12065755
• Inman SB-431542 is a potent and specific inhibitor of transforming growth factor-β superfamily type I AVNreceptor-like kinase (ALK) receptors ALK4, ALK5, and ALK7. Mol.Pharmacol. 2002 PMID: 12065756