Human Fibronectin PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1918P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # P1H11
Product Specifications
Immunogen
Human plasma-derived Fibronectin
Specificity
Detects human Fibronectin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Fibronectin PE-conjugated Antibody
Detection of Fibronectin in HepG2 cells by Flow Cytometry
HepG2 (filled histogram) and Jurkat (open histogram) cells were stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Fibronectin in WS-1 Human Cell Line by Flow Cytometry.
WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Fibronectin PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line orWS‑1 human fetal skin fibroblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line orWS‑1 human fetal skin fibroblast cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Human Fibronectin PE-conjugated Antibody
Product Specific Notices for Human Fibronectin PE-conjugated Antibody
For research use only
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