Human Fibronectin Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1918F

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

FITC (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P1H11

View all Fibronectin Antibodies »

Product Specifications

Immunogen

Human plasma-derived Fibronectin

Specificity

Detects human Fibronectin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibronectin Fluorescein-conjugated Antibody

Detection of Fibronectin in HepG2 cells by Flow Cytometry

HepG2 (filled histogram) and Jurkat (open histogram) cells were stained with Mouse Anti-Human Fibronectin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1918F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Fibronectin antibody in WS-1 Human Cell Line antibody by Flow Cytometry.

Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry.

WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1918F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Fibronectin Fluorescein-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: WS‑1 human fetal skin fibroblast cell line

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Fibronectin

Human Fibronectin (FN) is a 210-220 kDa extracellular matrix protein that serves as a cornerstone for cell-matrix interactions. It is a sulfated glycoprotein 2055 amino acids (aa) in length. Along its length, it exhibits three types of repeating modules; twelve type I, two type II and 16 type III modules. There are more than 15 alternative splice variants, most in the C-terminal half of the molecule. FN circulates as a disulfide-linked homodimer, a product of hepatocytes. It also exists as a very large covalent multimer, likely the product of a wide variety of cell types. Within these multimers will be found FN-Fibrinogen, -Collagen 13 and -Mac2BP complexes. The multidomain nature of FN allows for binding of Fibrin, GAGs (HS and CS), Syndecans and Integrins alpha5 beta1 and alphaV beta3. Over aa 31-2386 (full-length), human FN shares 91% aa sequence identity with mouse FN.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Human Fibronectin Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fibronectin Fluorescein-conjugated Antibody

For research use only

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