Human Fibronectin APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1918A

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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P1H11

View all Fibronectin Antibodies »

Product Specifications

Immunogen

Human plasma-derived Fibronectin

Specificity

Detects human Fibronectin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibronectin APC-conjugated Antibody

Detection of Fibronectin in HepG2 cells by Flow Cytometry

HepG2 (filled histogram) and Jurkat (open histogram) cells were stained with Mouse Anti-Human Fibronectin APC-conjugated Monoclonal Antibody (Catalog # IC1918A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Fibronectin antibody in WS-1 Human Cell Line antibody by Flow Cytometry.

Detection of Fibronectin in WS-1 Human Cell Line by Flow Cytometry.

WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin APC-conjugated Monoclonal Antibody (Catalog # IC1918A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Fibronectin APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HepG2 huan hepatocellular carcinoma cell line orWS‑1 human fetal skin fibroblast cell line

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Human Fibronectin APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Fibronectin APC-conjugated Antibody

For research use only

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