Bio-Techne Spatial’s fully automated, same-section multiomics workflow enables spatial RNA and protein detection at subcellular resolution. The best-in-class hyperplex multiomics application leverages RNAscope™ HiPlex technology from Advanced Cell Diagnostics and the universal multiplex sequential immunofluorescence (seqIF™) application on the COMET™ platform from Lunaphore.
Combining both assays on COMET, we provide end-users with multiplex data without compromising panel flexibility or sensitivity. The combined workflow allows users to select any 12 RNA targets and up to 24 protein targets using off-the-shelf, non-conjugated primary antibodies. The assay is designed to be compatible with current procedures on COMET using a unique protease-free pretreatment to promote streamlined, spatial multiomic data acquisition with subcellular resolution.
Image legend: Human breast cancer: UBC (orange) RPL28 (pink) RPL5 (yellow) GAPDH (green) CK (blue) DAPI (white)
Same-Section Multiomics
Same-Section Multiomics
- Single workflow for co-detection of RNA and protein targets on the same tissue section
- Generate spatially resolved multiomic data at subcellular resolution
Fully-Automated Workflow
Fully-Automated Workflow
- Automated from target probe hybridization and protein detection to imaging
- Stain up to 4 slides in every run
Flexible Assay Design
Flexible Assay Design
- Use our extensive RNAscope probe catalog or design custom probes for RNAs
- Design custom panels
Protease-Free, Fully-Automated Multiomics Workflow
Featured Applications
Immunology
Immunology
- Assess cytokine profiles in inflamed tissues and identify the cellular source of inflammatory molecules
- Visualize gene signatures with cell-specific markers to assess activation states of immune cells
Immuno-oncology
Immuno-oncology
- Evaluate the tumor immune context for a better therapeutic outcome
- Study tumor heterogeneity by visualizing unique morphology and expression profiles
Immunity and Infectious Disease
Immunity and Infectious Disease
- Localization—visualizing infected cell types and tissues enables elucidation of viral transmission, pathogen-host interactions, and localization of hidden reservoirs
- Differential detection—targeting the sense or anti-sense strand enables detection and differentiation of virus during latent or active stages
- Coinfection detection—detect multiple viruses or co-infection
Cytokine Detection with Multiomics on COMET
Sample Type: FFPE human breast cancer
RNA: IL6 (green)
Protein: CD3 (yellow), Vimentin (orange), aSMA (blue)
Search Antibodies
Work with off-the-shelf, non-conjugated primary antibodies or quickly start using SPYRE Antibody Panels and detection reagents from Lunaphore.
Get Started Today. Speak With A Specialist.
Watch our webinar on profiling the tumor microenvironment through same-section fully automated spatial multiomics.
| Type | Component | Catalog # |
|---|---|---|
| Reagent Kits | RNAscope HiPlex Pro for COMET 4-plex, 20-slide Reagent Kit | 322085 |
| RNAscope HiPlex Pro for COMET 12-plex, 20-slide Reagent Kit | 322075 | |
| Negative Control Probe | RNAscope HiPlex12 CS Negative Control Probe | 324347 |
| Positive Control Probes | RNAscope HiPlex12 CS Positive Control Probe-Hs | 324317 |
| RNAscope HiPlex12 CS Positive Control Probe-Mm | 324437 | |
| RNAscope HiPlex12 CS Positive Control Probe-Rn | 324337 |
View product page for complete PDF ordering checklist for all required materials.