Integration of RNA in situ Hybridization and Sequential Immunofluorescence for Same Slide Fully Automated Multiomics Analysis of the Tumor Microenvironment

Spatial biology has transformed our understanding of the tumor microenvironment (TME) by enabling the study of tissue composition and intercellular interactions at a single-cell level while preserving spatial context. Hyperplex immunofluorescence (IF) techniques allow the simultaneous detection of multiple protein biomarkers, enabling immune cell profiling in the TME. Similarly, RNA in situ hybridization (ISH) techniques have enabled the detection of RNA biomarkers, such as soluble factors with high sensitivity and specificity. This poster demonstrates the successful implementation of the combined RNAscope™ ISH and seqIF™ protocols on the COMET™ platform and provides valuable insights into unique infiltrating immune cell populations and their activation states.