Webinar: Advancing Charge Variant Characterization of mAbs and ADCs using icIEF Fractionation and LC-MS

Watch our webinar on demand “Advancing Charge Variant Characterization of mAbs and ADCs using icIEF Fractionation and LC-MS.” 

Discover how Imaged capillary isoelectric focusing (icIEF) fractionation combined with mass spectrometry (MS) enables in-depth characterization of charge heterogeneity in monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs). These variants can significantly affect a product’s pharmacokinetics, efficacy, safety, and stability, making comprehensive charge profiling a critical aspect of biopharmaceutical development and quality control. 

Nicki Zhang (MilliporeSigma) discusses an integrated workflow to characterize mAbs and ADCs with varying levels of heterogeneity. She demonstrates how icIEF fractionation combined with MS enhances the resolution and interpretability of charge variant profiles to support formulation development, process optimization, and comparability studies. 

Ms. Nelly Luong (Technical University of Braunschweig) describes a workflow for a preparative fractionation method for the mAb Matuzumab that improves the sensitivity for subsequent peptide mapping using LC-MS. 

The webinar covers the following topics: 

• A robust, scalable, and MS platform-independent analytical strategy for addressing charge heterogeneity challenges in complex biologics
• An improved understanding of critical quality attributes to accelerate development timelines and strengthen regulatory submissions 

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