Webinar: Addressing the Challenges of Size and Purity Analysis for Fusion Proteins, ADCs, and AAVs using CE-SDS

Watch our webinar on demand, “Addressing the Challenges of Size and Purity Analysis for Fusion Proteins, ADCs and AAVs using CE-SDS.”

Chia Thach (Bio-Techne) highlights the latest advancements in CE-SDS using the Maurice platform, which has been gradually replacing manual SDS-PAGE methods for biotherapeutic characterization due to its simple, accurate and robust workflow.

Caroline Daniels (Shattuck Labs) describes a workflow using the Maurice system to separate structurally similar molecules based on the number of N-linked glycosylation sites. After evaluating both the Maurice CE-SDS Plus and Turbo CE-SDS cartridges, her team selected the Turbo cartridge for routine analysis to reduce run time while maintaining resolution.

This method is being used to support process development decisions to enrich for the bispecific protein as well as to support good manufacturing practices (GMP) release and stability. This strategy can be readily applied to other heterodimeric or bispecific glycoproteins, provided there are sufficient differences in glycosylation between the polypeptide chains.