Rabbit IgG PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0110
Key Product Details
Species Reactivity
Rabbit
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Rabbit IgG
Specificity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rabbit IgG PE-conjugated Antibody
Detection of Rabbit IgG Primary Antibody by Flow Cytometry
Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (MAB6944) or (B) Normal Rabbit IgG Control (AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) at 10 µL/106 cells.
Detection of Rabbit IgG Primary Antibody by Flow Cytometry
U937 cells were stained with Rabbit Anti-Human IFN alpha betaR1 Monoclonal Antibody (2452, filled histogram) or (B) Normal Rabbit IgG Control (AB-105-C, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) at 10 µL/106 cells.Applications for Rabbit IgG PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Reviewed Applications
Read 2 reviews rated 5 using F0110 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitution default not known
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Rabbit IgG PE-conjugated Antibody
Product Specific Notices for Rabbit IgG PE-conjugated Antibody
For research use only
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