Recombinant Human IL-15 Protein

R&D Systems, part of Bio-Techne | Catalog # 247-IL

Discontinued Product
247-IL has been discontinued. An alternative/replacement product is available: 247-ILB. View all IL-15 products.

Key Product Details

Source

E. coli

Accession #

P40933

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-15 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-15 protein
Asn49-Ser162

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn49

Predicted Molecular Mass

12.5 kDa

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.
The ED50 for this effect is typically 0.3-2.6 ng/mL.
The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).

Reviewed Applications

Read 1 review rated 5 using 247-IL in the following applications:

Formulation, Preparation and Storage

247-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gammac) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta/ gammac on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12-16). An important component of these functions is the ability of IL‑15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).

References

  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Grabstein, K. et al. (1994) Science 264:965.
  3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  5. Giri, J. et al. (1994) EMBO J. 13:2822.
  6. Dubois, S. et al. (2002) Immunity 17:537.
  7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  10. Bergamaschi, C. et al. (2012) Blood 120:e1.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
  13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  18. Barra, N.G. et al. (2010) Obesity 18:1601.

Long Name

Interleukin 15

Alternate Names

IL15

Entrez Gene IDs

3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)

Gene Symbol

IL15

UniProt

P40933

Additional IL-15 Products

Product Documents for Recombinant Human IL-15 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-15 Protein

For research use only

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