Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

Name: Proteome Profiler Human Cytokine Array Kit
Brand: R&D Systems
SKU: ARY005B
Rating: 5 (12 reviews)

The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.

Proteome Profiler Human Cytokine Array Kit Best Seller

R&D Systems, part of Bio-Techne | Catalog # ARY005B

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Product Datasheet / COA / SDS

Contains 4 membranes - each spotted in duplicate with 36 different cytokine antibodies

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Product Summary
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Product Summary for Proteome Profiler Human Cytokine Array Kit

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.

Troubleshooting Guide

Key Benefits

Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
Requires no specialized equipment
Compatible with LI-COR* and chemiluminescence detection

*If using a LI-COR, additional reagents and protocol modifications are required. Refer to protocol.

Principle of the Assay

The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

Kit Contents

Rectangular 4-Well Multi-dish
Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
Array Buffer 4
Array Buffer 5
Chemi Reagent 1
Chemi Reagent 2
Detection Antibody Cocktail, Human Cytokine Array
Streptavidin-HRP
Transparency Overlay Template
Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
C5a	IL-4	IL-27
CD40 Ligand	IL-5	IL-32 alpha
G-CSF	IL-6	CXCL10/IP-10
GM-CSF	IL-8	CXCL11/I-TAC
CXCL1/GRO alpha	IL-10	CCL2/MCP-1
CCL1/I-309	IL-12 p70	MIF
ICAM-1	IL-13	MIP-1 alpha/MIP-1 beta
IFN-gamma	IL-16	CCL5/RANTES
IL-1 alpha	IL-17	CXCL12/SDF-1
IL-1 beta	IL-17E	Serpin E1/PAI-1
IL-1ra	IL-18	TNF-alpha
IL-2	IL-21	TREM-1

Assays for Analytes represented in the Human Cytokine Array Kit

Analyte	Quantikine^® ELISA Kits	Quantikine^® HS (High Sensitivity) ELISA Kits	QuantiGlo^® ELISA Kits	DuoSet^® ELISA Development Systems
C5a				DY2037
CD40 Ligand	DCDL40			DY617
G-CSF	DCS50	HSTCS0		DY214
GM-CSF	DGM00	HSGM0		DY215
CXCL1/GRO alpha	DGR00			DY275
CCL1/I-309				DY272
ICAM-1	DCIM00			DY720
IFN-gamma	DIF50C			DY285B
IL-1 alpha	DLA50			DY200
IL-1 beta	DLB50	HSLB00C	QLB00B	DY201
IL-1ra	DRA00B			DY280
IL-2	D2050		Q2000B	DY202
IL-4	D4050	HS400		DY204
IL-5	D5000B			DY205
IL-6	D6050B	HS600B	Q6000B	DY206
CXCL8/IL-8	D8000C	HS800	Q8000B	DY208
IL-10	D1000B	HS100C	Q1000	DY217B
IL-12 p70	D1200	HS120		DY1270
IL-13	D1300B			DY213
IL-16	D1600			DY316
IL-17	D1700			DY317
IL-17E
IL-18	7620
IL-21
IL-27				DY2526
IL-32 alpha
CXCL10/IP-10	DIP100			DY266
CXCL11/I-TAC	DCX110			DY672
CCL2/MCP-1	DCP00			DY279
MIF	DMF00B			DY289
CCL3/MIP-1 alpha	DMA00			DY270
CCL4/MIP-1 beta	DMB00			DY271
Serpin E1/PAI-1	DSE100, DTSE100			DY1786
CCL5/RANTES	DRN00B			DY278
CXCL12/SDF-1	DSA00			DY350
TNF-alpha	DTA00C	HSTA00D	QTA00B	DY210
TREM-1	DTRM10B			DY1278B

Scientific Data Images for Proteome Profiler Human Cytokine Array Kit

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.

The amount of cell culture supernate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 1A: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL LPS for 4 hours, then 5mM Nigericin (Tocris, Catalog # 4312) for 1 hour (500 µL of cell culture supernate, 10 minute exposure). 1B: SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (500 µL of cell culture supernate, 10 minute exposure).

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates.

The amount of cell lysate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 2A: SW1353 human chondrosarcoma cells were untreated or treated with 5 ng/mL recombinant human IL-1 alpha (R&D Systems, Catalog # 200-LA) and 10 ng/mL recombinant human OSM (R&D Systems, Catalog # 295-OM) for 24 hours (200 µg of cell lysate, 5 minute exposure). 2B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, then 1 µg/mL LPS for 16 hours (200 µg of cell lysate, 5 minute exposure).

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples.

200 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.

1. ICAM-1/CD54
2. MIF
3. Complment Component C5/C5a
4. CXCL1/GRO alpha
5. IL-8
6. IL-16
7. IL-18
8. CCL5/RANTES
9. IL-1ra/IL-1F3
10. Serpin E1/PAI-1

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex^® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.