Human TREM-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTRM10B
Discontinued Product
DTRM10B has been discontinued.
View all TREM-1 products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL)
Sensitivity
30.6 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human TREM-1 Quantikine ELISA Kit
The Quantikine Human TREM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TREM-1 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TREM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring TREM-1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Reactivity
Human
Specificity
Natural and recombinant human TREM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 410 | 1212 | 2424 | 439 | 1215 | 2354 |
| Standard Deviation | 21.3 | 50.7 | 87.9 | 32.7 | 70.4 | 166 |
| CV% | 5.2 | 4.2 | 3.6 | 7.4 | 5.8 | 7.1 |
Recovery for Human TREM-1 Quantikine ELISA Kit
The recovery of TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 109 | 97-119 |
| EDTA Plasma (n=4) | 98 | 86-108 |
| Heparin Plasma (n=4) | 99 | 85-109 |
| Serum (n=4) | 98 | 87-110 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of TREM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TREM-1 Quantikine ELISA Kit
Human TREM-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: TREM-1
The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Alternate Names
CD354, TREM1
Gene Symbol
TREM1
Additional TREM-1 Products
Product Documents for Human TREM-1 Quantikine ELISA Kit
Product Specific Notices for Human TREM-1 Quantikine ELISA Kit
For research use only
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