Human CXCL10/IP-10 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DIP100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (75 µL), EDTA Plasma (75 µL), Heparin Plasma (75 µL), Saliva (10 µL), Urine (100 µL)
Sensitivity
4.46 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human CXCL10/IP-10 Quantikine ELISA Kit
The Quantikine Human IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IP-10 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IP-10.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IP-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human IP-10 in this assay. No medical histories were available for the donors
used in this study.
ND=Non-detectable
THP-1 human acute monocyte leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were stimulated with 1.0 μg/mL recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells were incubated for an additional 18 hours. An aliquot of the cell culture supernate was removed, diluted 600-fold and assayed, and measured 164,640 pg/mL.
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serum (n=60) | 89 | 38-361 |
| EDTA plasma (n=35) | 96 | 47-382 |
| Heparin plasma (n=35) | 110 | 52-450 |
| Saliva (n=5) | 729 | 292-1340 |
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Urine (n=18) | 17.2 | 67 | ND-49.7 |
Cell Culture Supernates:
Human peripheral blood cells (1 x 106
cells/mL) were cultured in DMEM supplemented with
5% fetal bovine serum, 50 mM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with
10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of
human IP-10.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 29,774 | 21,900 |
| Stimulated | 18,456 | 11,091 |
THP-1 human acute monocyte leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were stimulated with 1.0 μg/mL recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells were incubated for an additional 18 hours. An aliquot of the cell culture supernate was removed, diluted 600-fold and assayed, and measured 164,640 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 70.3 | 174 | 342 | 80.7 | 194 | 362 |
| Standard Deviation | 2.07 | 5.15 | 10.74 | 7.91 | 13.48 | 24.27 |
| CV% | 2.9 | 3.0 | 3.1 | 9.8 | 6.9 | 6.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 67.4 | 168 | 334 | 70.7 | 182 | 366 |
| Standard Deviation | 3.12 | 4.99 | 11.15 | 6.23 | 11.12 | 18.93 |
| CV% | 4.6 | 3.0 | 3.3 | 8.8 | 6.1 | 5.2 |
Recovery for Human CXCL10/IP-10 Quantikine ELISA Kit
The recovery of IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 88-101 |
| EDTA Plasma (n=5) | 99 | 90-109 |
| Heparin Plasma (n=5) | 99 | 87-113 |
| Serum (n=5) | 99 | 88-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IP-10 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL10/IP-10 Quantikine ELISA Kit
Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD5K Standard Curve
Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD6Q Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Human CXCL10/IP-10 Quantikine ELISA Kit
Product Specific Notices for Human CXCL10/IP-10 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.